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Human Protein-L-isoaspartate (PCMT1) ELISA Kit

Human Protein-L-isoaspartate (PCMT1) ELISA Kit

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Specification

Product name Human Protein-L-isoaspartate (PCMT1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein-L-isoaspartate (PCMT1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCMT1 in samples. An antibody specific for PCMT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCMT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCMT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCMT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PCMT1; L-isoaspartyl/D-aspartyl methyltransferase; OTTHUMP00000017400; protein-L-isoaspartate (D-aspartate) O-methyltransferase 1

Product Properties

Kit components • Human Protein-L-isoaspartate microplate
• Human Protein-L-isoaspartate standard
• Human Protein-L-isoaspartate detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein-L-isoaspartate (PCMT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCMT1. No significant cross-reactivity or interference between Human PCMT1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Three classes of protein carboxyl methyltransferases, distinguished by their methyl-acceptor substrate specificity, have been found in prokaryotic and eukaryotic cells. The type II enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine to the free carboxyl groups of D-aspartyl and L-isoaspartyl residues. These methyl-accepting residues result from the spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues and represent sites of covalent damage to aging proteins PCMT1 (EC 2.1.1.77) is a protein repair enzyme that initiates the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form.
Gene ID 5110
Alternative PCMT1; L-isoaspartyl/D-aspartyl methyltransferase; OTTHUMP00000017400; protein-L-isoaspartate (D-aspartate) O-methyltransferase 1
Accession P22061

Image & description

Fig.1. Human Protein-L-isoaspartate (PCMT1) Standard Curve.

Fig.1. Human Protein-L-isoaspartate (PCMT1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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