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Human Protein kinase C delta type (PRKCD) ELISA Kit

Human Protein kinase C delta type (PRKCD) ELISA Kit

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Specification

Product name Human Protein kinase C delta type (PRKCD) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein kinase C delta type (PRKCD) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKCD in samples. An antibody specific for PRKCD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKCD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKCD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKCD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRKCD; MAY1; MGC49908; PKCD; nPKC-delta; protein kinase C delta VIII

Product Properties

Kit components • Human Protein kinase C delta type microplate
• Human Protein kinase C delta type standard
• Human Protein kinase C delta type detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein kinase C delta type (PRKCD) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRKCD. No significant cross-reactivity or interference between Human PRKCD and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The genes encoding the protein kinase C enzymes are widely distributed, e.g., PRKCA on chromosome 17, PRKCB1 on 16, and PRKCG on 19. Although the sequence homology among the PRKC family of genes is extensive, the pattern of expression varies among tissues. For example, the delta polypeptide appears to be the major isoform expressed in mouse hematopoietic cells. Mischak et al. (1991) isolated and characterized the mouse Prkcd gene.Aris et al. (1993) found that PKC-delta underwent calcium-independent autophosphorylation in the presence of phosphatidylserine and diacylglycerol. Diacylglycerol was an absolute requirement for PKC-delta activation. This and other cofactor and substrate requirements distinguished human PKC-delta from its mouse homolog. Organism species: Homo sapiens (Human)
Gene ID 5580
Alternative PRKCD; MAY1; MGC49908; PKCD; nPKC-delta; protein kinase C delta VIII
Accession Q05655

Image & description

Fig.1. Human Protein kinase C delta type (PRKCD) Standard Curve.

Fig.1. Human Protein kinase C delta type (PRKCD) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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