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Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit

Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit

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Specification

Product name Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX3 in samples. An antibody specific for TMX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMX3; FLJ20793; KIAA1830; PDIA13; TXNDC10; protein disulfide isomerase family A; member 13; thioredoxin domain containing 10

Product Properties

Kit components • Human Protein disulfide-isomerase TMX3 microplate
• Human Protein disulfide-isomerase TMX3 standard
• Human Protein disulfide-isomerase TMX3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMX3. No significant cross-reactivity or interference between Human TMX3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The TMX3 gene encodes a protein of 454 amino acid residues that contains a predicted N-terminal signal sequence, a single domain with sequence similarity to thioredoxin and a CGHC active site sequence, a potential transmembrane domain, and a C-terminal KKKD tetrapeptide sequence that matches the classical KKXX-type consensus sequence for ER retrieval of type I transmembrane proteins. Endogenous TMX3 contains endoglycosidase H-sensitive glycans, localizes to the ER by immunofluorescence microscopy, and is present in the membrane fraction after alkaline extraction of the ER luminal content. The TMX3 transcript is found in a variety of tissues and is not up-regulated by the unfolded protein response.
Gene ID 54495
Alternative TMX3; FLJ20793; KIAA1830; PDIA13; TXNDC10; protein disulfide isomerase family A; member 13; thioredoxin domain containing 10
Accession Q96JJ7

Image & description

Fig.1. Human Protein disulfide-isomerase TMX3 (TMX3) Standard Curve.

Fig.1. Human Protein disulfide-isomerase TMX3 (TMX3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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