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Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit

Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit

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Specification

Product name Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA2 in samples. An antibody specific for PDIA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDIA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PDIA2; LA16c-314G4.2; PDA2; PDI; PDIP; PDIR; Rho GDP dissociation inhibitor gamma; pancreatic protein disulfide isomerase; protein disulfide isomerase A2; protein disulfide isomerase; pancreatic; protein disul

Product Properties

Kit components • Human Protein disulfide-isomerase A2 microplate
• Human Protein disulfide-isomerase A2 standard
• Human Protein disulfide-isomerase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Protein disulfide-isomerase A2 (PDIA2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDIA2. No significant cross-reactivity or interference between Human PDIA2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions. Desilva et al. (1996) cloned PDIP from an insulinoma subtraction cDNA library. The deduced 511-amino acid protein has a calculated molecular mass of about 56.6 kD and contains 2 thioredoxin-like catalytic sites, a C-terminal ER retention sequence (KEEL), and 3 potential N-glycosylation sites. PDIP shares about 46% identity with bovine, mouse, rabbit, and human PDIs . Northern blot analysis detected a 2.0-kb transcript expressed exclusively in pancreas.
Gene ID 64714
Alternative PDIA2; LA16c-314G4.2; PDA2; PDI; PDIP; PDIR; Rho GDP dissociation inhibitor gamma; pancreatic protein disulfide isomerase; protein disulfide isomerase A2; protein disulfide isomerase; pancreatic; protein disul
Accession Q13087

Image & description

Fig.1. Human Protein disulfide-isomerase A2 (PDIA2) Standard Curve.

Fig.1. Human Protein disulfide-isomerase A2 (PDIA2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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