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Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit

Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit

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Specification

Product name Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit employs a two-site sandwich ELISA to quantitate ADPRH in samples. An antibody specific for ADPRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADPRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADPRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADPRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ADPRH; ARH1; ADP-ribose-L-arginine cleaving enzyme

Product Properties

Kit components • Human [Protein ADP-ribosylarginine] hydrolase microplate
• Human [Protein ADP-ribosylarginine] hydrolase standard
• Human [Protein ADP-ribosylarginine] hydrolase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADPRH. No significant cross-reactivity or interference between Human ADPRH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The deduced human ADPRH protein has 357 amino acids and an estimated molecular mass of 39.5 kD. Human ADPRH shows 83% amino acid identity to rat Adprh and 82% identity to mouse Adprh. The authors found that the mouse and rat Adprh sequences contain 5 conserved cysteine residues; the human sequence contains only 4 cysteines, with cys108 in rat corresponding to ser103 in human. Expression of recombinant wildtype and mutant rat and human ADPRH proteins in E. coli and assessment of their DTT requirements for hydrolase activity demonstrated that cys108 is responsible for the DTT dependence of rat Adprh. Northern blot analysis detected a 4.1-kb ADPRH transcript in various human tissues, including fetal and adult brain.
Gene ID 141
Alternative ADPRH; ARH1; ADP-ribose-L-arginine cleaving enzyme
Accession P54922

Image & description

Fig.1. Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) Standard Curve.

Fig.1. Human [Protein ADP-ribosylarginine] hydrolase (ADPRH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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