| Product name | Human Proteasome activator complex subunit 3 (PSME3) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Proteasome activator complex subunit 3 (PSME3) ELISA Kit employs a two-site sandwich ELISA to quantitate PSME3 in samples. An antibody specific for PSME3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSME3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSME3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSME3 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PSME3; Ki; PA28-gamma; PA28G; REG-GAMMA; 11S regulator complex gamma subunit; Ki antigen; Ki nuclear autoantigen; activator of multicatalytic protease subunit 3; proteasome activator 28-gamma; proteasome activa |
| Kit components | • Human Proteasome activator complex subunit 3 microplate • Human Proteasome activator complex subunit 3 standard • Human Proteasome activator complex subunit 3 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Proteasome activator complex subunit 3 (PSME3) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSME3. No significant cross-reactivity or interference between Human PSME3 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. |
| Gene ID | 10197 |
| Alternative | PSME3; Ki; PA28-gamma; PA28G; REG-GAMMA; 11S regulator complex gamma subunit; Ki antigen; Ki nuclear autoantigen; activator of multicatalytic protease subunit 3; proteasome activator 28-gamma; proteasome activa |
| Accession | P61289 |
Fig.1. Human Proteasome activator complex subunit 3 (PSME3) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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