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Human Prostaglandin E synthase (PTGES) ELISA Kit

Human Prostaglandin E synthase (PTGES) ELISA Kit

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Specification

Product name Human Prostaglandin E synthase (PTGES) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Prostaglandin E synthase (PTGES) ELISA Kit employs a two-site sandwich ELISA to quantitate PTGES in samples. An antibody specific for PTGES has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTGES present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGES is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGES bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTGES; MGC10317; MGST-IV; MGST1-L1; MGST1L1; MPGES; PGES; PIG12; PP102; PP1294; TP53I12; mPGES-1; MGST1-like 1; glutathione S-transferase 1-like 1; microsomal glutathione S-transferase 1-like 1; microsomal pr

Product Properties

Kit components • Human Prostaglandin E synthase microplate
• Human Prostaglandin E synthase standard
• Human Prostaglandin E synthase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Prostaglandin E synthase (PTGES) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTGES. No significant cross-reactivity or interference between Human PTGES and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Prostaglandin E synthase is an enzyme involved in eicosanoid and glutathione metabolism, a member of MAPEG family. It generates prostaglandin E (PGE) from prostaglandin H2.The synthase generating PGE2 is a membrane-associated protein. Prostaglandin E synthase is a glutathione-dependent prostaglandin E synthase. The expression of this gene has been shown to be induced by proinflammatory cytokine interleukin 1 beta (IL1B). Its expression can also be induced by tumor suppressor protein TP53, and may be involved in TP53 induced apoptosis.The authors grouped the MGST1L1 protein with other members of the MAPEG (membrane-associated proteins in eicosanoid and glutathione metabolism) superfamily, which includes MGST2 , MGST3 , LTC4S, and FLAP (ALOX5AP).
Gene ID 9536
Alternative PTGES; MGC10317; MGST-IV; MGST1-L1; MGST1L1; MPGES; PGES; PIG12; PP102; PP1294; TP53I12; mPGES-1; MGST1-like 1; glutathione S-transferase 1-like 1; microsomal glutathione S-transferase 1-like 1; microsomal pr
Accession O14684

Image & description

Fig.1. Human Prostaglandin E synthase (PTGES) Standard Curve.

Fig.1. Human Prostaglandin E synthase (PTGES) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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