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Human Prosaposin (PSAP) ELISA Kit

Human Prosaposin (PSAP) ELISA Kit

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Specification

Product name Human Prosaposin (PSAP) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Prosaposin (PSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PSAP in samples. An antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSAP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PSAP; FLJ00245; GLBA; MGC110993; SAP1; sphingolipid activator protein-1

Product Properties

Kit components • Human Prosaposin microplate
• Human Prosaposin standard
• Human Prosaposin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Prosaposin (PSAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PSAP. No significant cross-reactivity or interference between Human PSAP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO[S]teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases.
Gene ID 5660
Alternative PSAP; FLJ00245; GLBA; MGC110993; SAP1; sphingolipid activator protein-1
Accession P07602

Image & description

Fig.1. Human Prosaposin (PSAP) Standard Curve.

Fig.1. Human Prosaposin (PSAP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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