|Product name||Human Procollagen I N-terminal peptide (PINP) ELISA Kit|
|Applications notes||This Human Procollagen I N-terminal peptide (PINP) ELISA Kit employs a two-site sandwich ELISA to quantitate PINP in samples. An antibody specific for PINP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPINP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PINP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PINP bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Procollagen I N-terminal peptide microplate
• Human Procollagen I N-terminal peptide standard
• Human Procollagen I N-terminal peptide detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Procollagen I N-terminal peptide (PINP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PINP. No significant cross-reactivity or interference between Human PINP and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The PINP molecule is similar to PIIINP consisting of three distinct structural domains: Col 1 is on the aminoterminal side of the molecule, while Col 2 and Col 3 are situated on the middle of the helically structured molecule (Kühn et al. 1982). The PINP molecule has a molecular mass of 35 000 and is cleared by scavenger receptors in liver endothelial cells (Melkko et al. 1994). PINP often occurs in circulation in two forms of different molecular sizes. One is identical to the trimeric authentic antigen (intact PINP) whereas the other consists of smaller forms of PINP, resembling a single domain of the proα1(I) chain of PINP and is probably a degradation product of type I procollagen or I pN-collagen. Thus, an assay of intact PINP rather than total PINP appears to be more sensitive in detecting changes in the rate of type I collagen synthesis (Melkko et al. 1996, Risteli & Risteli 1999).|
Fig.1. Human Procollagen I N-terminal peptide (PINP) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.