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Human Prickle-like protein 2 (PRICKLE2) ELISA Kit

Human Prickle-like protein 2 (PRICKLE2) ELISA Kit

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Specification

Product name Human Prickle-like protein 2 (PRICKLE2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Prickle-like protein 2 (PRICKLE2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRICKLE2 in samples. An antibody specific for PRICKLE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRICKLE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRICKLE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRICKLE2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRICKLE2; DKFZp686D143; DKFZp686H1748; DKFZp686M031; prickle-like 2

Product Properties

Kit components • Human Prickle-like protein 2 microplate
• Human Prickle-like protein 2 standard
• Human Prickle-like protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Prickle-like protein 2 (PRICKLE2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRICKLE2. No significant cross-reactivity or interference between Human PRICKLE2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background By searching an EST database for sequences similar to those of Drosophila and Xenopus Prickle, Katoh and Katoh (2003) identified human PRICKLE2. The deduced 844-amino acid protein contains an N-terminal PET domain followed by 3 LIM domains and a C-terminal prickle homology domain. PRICKLE2 shares 51.9% overall identity with PRICKLE1 and 79.3% identity within the N-terminal PET and LIM domains. EST database analysis revealed that PRICKLE1 and PRICKLE2 are coexpressed in brain, eye, and testis; additionally, PRICKLE2 is expressed in fetal brain, adult cartilage, pancreatic islet, gastric cancer, and uterine tumor.Katoh and Katoh (2003) determined that the PRICKLE2 gene contains at least 8 exons and that the 5-prime untranslated region is interrupted by intron 1.
Gene ID 166336
Alternative PRICKLE2; DKFZp686D143; DKFZp686H1748; DKFZp686M031; prickle-like 2
Accession Q7Z3G6

Image & description

Fig.1. Human Prickle-like protein 2 (PRICKLE2) Standard Curve.

Fig.1. Human Prickle-like protein 2 (PRICKLE2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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