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Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit

Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit

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Specification

Product name Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit employs a two-site sandwich ELISA to quantitate PARG in samples. An antibody specific for PARG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PARG; FLJ60456; PARG99; poly(ADP-ribose) glycohydrolase 60 kDa isoform

Product Properties

Kit components • Human Poly microplate
• Human Poly standard
• Human Poly detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Poly (ADP-ribose) glycohydrolase (PARG) ELISA Kit has high sensitivity and excellent specificity for detection of Human PARG. No significant cross-reactivity or interference between Human PARG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PARG is a protein involved in a number of cellular processes involving mainly DNA repair and programmed cell death.RP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress . This protein can be cleaved by many ICE-like caspases in vitroand is one of the main cleavage targets of caspase-3 in vivo . In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) . PARP1 is a chromatin-associated enzyme, poly-ADP ribose polymerase-1, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. PARP1 has a role in repair of single-stranded DNA (ssDNA) breaks.
Gene ID 670
Alternative PARG; FLJ60456; PARG99; poly(ADP-ribose) glycohydrolase 60 kDa isoform
Accession Q86W56

Image & description

Fig.1. Human Poly (ADP-ribose) glycohydrolase (PARG) Standard Curve.

Fig.1. Human Poly (ADP-ribose) glycohydrolase (PARG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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