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Human Plasma serine protease inhibitor (SERPINA5) ELISA Kit

Human Plasma serine protease inhibitor (SERPINA5) ELISA Kit

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Specification

Product name Human Plasma serine protease inhibitor (SERPINA5) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Plasma serine protease inhibitor (SERPINA5) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINA5 in samples. An antibody specific for SERPINA5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINA5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SERPINA5; PAI3; PCI; PLANH3; PROCI; plasminogen activator inhibitorI; plasminogen activator inhibitor-3; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 5

Product Properties

Kit components • Human Plasma serine protease inhibitor microplate
• Human Plasma serine protease inhibitor standard
• Human Plasma serine protease inhibitor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Plasma serine protease inhibitor (SERPINA5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SERPINA5. No significant cross-reactivity or interference between Human SERPINA5 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Protein C inhibitor (PCI, SERPINA5) is a serine protease inhibitor (serpin) which limits the expression of protein C.Suzuki et al. (1987) determined the sequence of cDNA for human protein C inhibitor (PCI). A high degree of homology of the deduced amino acid sequence to that of other known inhibitors clearly demonstrated that protein C inhibitor is a member of the superfamily of serine protease inhibitors including alpha-1-antichymotrypsin (AACT), alpha-1-antitrypsin (AAT; PI), antithrombin III (AT3), and angiotensinogen (AGT). Protein C inhibitor is sometimes referred to as plasminogen activator inhibitor-3 (PAI3) because it also inhibits plasminogen activators (Meijers and Chung, 1991). Plasminogen activator inhibitor-1 (PAI1) has structural similarities to PCI.
Gene ID 338050
Alternative SERPINA5; PAI3; PCI; PLANH3; PROCI; plasminogen activator inhibitorI; plasminogen activator inhibitor-3; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 5
Accession Q9N2I2

Image & description

Fig.1. Human Plasma serine protease inhibitor (SERPINA5) Standard Curve.

Fig.1. Human Plasma serine protease inhibitor (SERPINA5) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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