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Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit

Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit

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Specification

Product name Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PGAM1 in samples. An antibody specific for PGAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PGAM1; RP11-452K12.8; PGAM-B; PGAMA; BPG-dependent PGAM 1; phosphoglycerate mutase 1; phosphoglycerate mutase A; nonmuscle form

Product Properties

Kit components • Human Phosphoglycerate mutase 1 microplate
• Human Phosphoglycerate mutase 1 standard
• Human Phosphoglycerate mutase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PGAM1. No significant cross-reactivity or interference between Human PGAM1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphoglyceric acid mutase is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The fact that the PGAMA and GOT1 loci are linked in the mouse supports the assignment of PGAMA to human chromosome 10. PGAM is a dimeric enzyme containing, in different tissues, different proportions of a muscle (MM) isozyme, a brain (BB) isozyme, and a hybrid form (MB). Electrophoresis of normal adult human muscle PGAM shows marked predominance of the MM band with only faint BB and MB bands. In most other human tissues, including brain, liver, erythrocytes, and leukocytes, PGAM-BB is the only demonstrable isozyme. In cardiac muscle extracts, all 3 bands are seen, although PGAM-MM predominates.
Gene ID 5223
Alternative PGAM1; RP11-452K12.8; PGAM-B; PGAMA; BPG-dependent PGAM 1; phosphoglycerate mutase 1; phosphoglycerate mutase A; nonmuscle form
Accession P18669

Image & description

Fig.1. Human Phosphoglycerate mutase 1 (PGAM1) Standard Curve.

Fig.1. Human Phosphoglycerate mutase 1 (PGAM1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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