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Human Phosphoglucomutase-2 (PGM2) ELISA Kit

Human Phosphoglucomutase-2 (PGM2) ELISA Kit

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Specification

Product name Human Phosphoglucomutase-2 (PGM2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Phosphoglucomutase-2 (PGM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PGM2 in samples. An antibody specific for PGM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PGM2; FLJ10983; MSTP006; glucose phosphomutase 2

Product Properties

Kit components • Human Phosphoglucomutase-2 microplate
• Human Phosphoglucomutase-2 standard
• Human Phosphoglucomutase-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Phosphoglucomutase-2 (PGM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PGM2. No significant cross-reactivity or interference between Human PGM2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The PGM2 protein shares about 20% identity with mammalian PGM1. Sequence analysis suggested that PGM2 is a cytosolic protein. Quantitative RT-PCR of mouse tissues detected the highest expression levels of PGM2 in lung, spleen, and thymus. PGM2 acted more than 10-fold better as a phosphopentomutasethan as a phosphoglucomutase. PGM2 may play a role in congenital immunodeficiencies. phosphopentomutase uses ribose 1-phosphate and deoxyribose 1-phosphate, which are formed by purine nucleoside phosphorylase and uridine phosphorylase, and that the absence of phosphopentomutase should result in the accumulation of ribose 1-phosphate and deoxyribose 1-phosphate and therefore in a functional block of purine nucleoside phosphorylase.
Gene ID 55276
Alternative PGM2; FLJ10983; MSTP006; glucose phosphomutase 2
Accession Q96G03

Image & description

Fig.1. Human Phosphoglucomutase-2 (PGM2) Standard Curve.

Fig.1. Human Phosphoglucomutase-2 (PGM2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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