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Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit

Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit

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Specification

Product name Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit employs a two-site sandwich ELISA to quantitate PIK3CB in samples. An antibody specific for PIK3CB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIK3CB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIK3CB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIK3CB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PIK3CB; DKFZp779K1237; MGC133043; P110BETA; PI3K; PI3KBETA; PIK3C1; PI3-kinase p110 subunit beta; PtdIns-3-kinase p110; catalytic phosphatidylinositol 3-kinase beta

Product Properties

Kit components • Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform microplate
• Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform standard
• Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PIK3CB. No significant cross-reactivity or interference between Human PIK3CB and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit. The predicted 1,070-amino acid protein, called p110-beta by them, is 42% identical to that of bovine p110. Northern blot analysis revealed that the major 4.8-kb p110-beta transcript was expressed in several human and rodent cell lines, as well as in all mouse tissues tested. Minor larger transcripts were detected in some tissues and cell lines.
Gene ID 5291
Alternative PIK3CB; DKFZp779K1237; MGC133043; P110BETA; PI3K; PI3KBETA; PIK3C1; PI3-kinase p110 subunit beta; PtdIns-3-kinase p110; catalytic phosphatidylinositol 3-kinase beta
Accession P42338

Image & description

Fig.1. Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) Standard Curve.

Fig.1. Human Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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