|Product name||Human Peroxisomal membrane protein 2 (PXMP2) ELISA Kit|
|Applications notes||This Human Peroxisomal membrane protein 2 (PXMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP2 in samples. An antibody specific for PXMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Alternative||PXMP2; FLJ54922; PMP22;|
|Kit components||• Human Peroxisomal membrane protein 2 microplate
• Human Peroxisomal membrane protein 2 standard
• Human Peroxisomal membrane protein 2 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Peroxisomal membrane protein 2 (PXMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PXMP2. No significant cross-reactivity or interference between Human PXMP2 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||PMP22 is a major component of peroxisomal membranes in mammals.It seems to be involved in pore forming activity and may contribute to the unspecific permeability of the organelle membrane. PMP22 is synthesized on free cytosolic ribosomes and then directed to the peroxisome membrane by specific targeting information. Previous studies in rats revealed that PMP22 contains one distinct peroxisomal membrane targeting signal in the amino-terminal cytoplasmic tail. Amino acid sequence alignment of rat and human protein revealed 77% identity including a high conservation of several protein motifs.Human as well as rat PMP22 contains two distinct and nonoverlapping peroxisomal membrane targeting signals, one in the amino-terminal and the other in the carboxyl-terminal protein region.|
|Alternative||PXMP2; FLJ54922; PMP22;|
Fig.1. Human Peroxisomal membrane protein 2 (PXMP2) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.