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Human Peripherin (PRPH) ELISA Kit

Human Peripherin (PRPH) ELISA Kit

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Product name Human Peripherin (PRPH) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Peripherin (PRPH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPH in samples. An antibody specific for PRPH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRPH; NEF4; PRPH1; neurofilament 4 (57kD)

Product Properties

Kit components • Human Peripherin microplate
• Human Peripherin standard
• Human Peripherin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Peripherin (PRPH) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRPH. No significant cross-reactivity or interference between Human PRPH and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Peripherin, a type III intermediate filament protein, was initially described by Portier et al. (1984) as a cytoskeletal protein present in neurons of the mammalian peripheral nervous system (hence, the name) and in cultured neuroblastoma cells. The amino-terminal region contained potential serine phosphorylation sites. Northern blot analysis detected a corresponding mRNA in ganglia of the peripheral nervous system, including the superior cervical ganglion, ciliary ganglion, and dorsal root ganglion. In the central nervous system, expression was detected in motor nuclei of cranial nerves and ventral horn neurons. Expression was induced by nerve growth factor.
Gene ID 5630
Alternative PRPH; NEF4; PRPH1; neurofilament 4 (57kD)
Accession P41219

Image & description

Fig.1. Human Peripherin (PRPH) Standard Curve.

Fig.1. Human Peripherin (PRPH) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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