| Product name | Human PDGF-BB protein |
| Sequence | Amino acid sequence derived from Human PDGF-BB (Ser82-Thr190) (P01127) was expressed. |
| Applications | No tag |
| Applications notes | Human |
| Activity | Measured in a cell proliferation assay using BaIb/c 3T3. The ED50 for this effect is typically 2.588 ng/mL. The specific activity of recombinant Human PDGF-BB is approximately >3.86 x 105 IU/mg. |
| Protein length | The recombinant Human PDGF-BB Protein consists of 109amino acids and predicts a molecular mass of 12.3 kDa. |
| Preparation method | E.coli |
| Purity | > 98 % as determined by SDS-PAGE |
| Molecular Formula | 12.3 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Molecular weight | 12.3 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Platelet derived growth factor (PDGF) is a potent mitogen and chemoattractant for mesenchymal and osteogenic cells and stimulates angiogenic molecules which play an essential role in bone regeneration. PDGF-BB is a member of PDGF family, which promotes cell proliferation, survival and migration, through binding to the tyrosine kinase PDGF receptor. PDGF-BB exerts beneficial effects on haemorrhagic shock, which are closely related to targeting CX43 to improve vascular reactivity and haemodynamics. PDGF-BB functions in corporal cavernosum smooth muscle cells (CCSMCs) via binding to PDGFR. |
| Gene ID | 5155 |
| Accession | P01127 |
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