| Product name | Human Pancreas transcription factor 1 subunit alpha (PTF1A) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Pancreas transcription factor 1 subunit alpha (PTF1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PTF1A in samples. An antibody specific for PTF1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTF1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTF1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTF1A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | PTF1A; PTF1-p48; bHLHa29; bHLH transcription factor p48; class bHLH protein PTF1A; exocrine pancreas-specific transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; pancreas transcr |
| Kit components | • Human Pancreas transcription factor 1 subunit alpha microplate • Human Pancreas transcription factor 1 subunit alpha standard • Human Pancreas transcription factor 1 subunit alpha detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Pancreas transcription factor 1 subunit alpha (PTF1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTF1A. No significant cross-reactivity or interference between Human PTF1A and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Pancreas transcription factor 1 subunit alpha is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers.Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers. |
| Gene ID | 256297 |
| Alternative | PTF1A; PTF1-p48; bHLHa29; bHLH transcription factor p48; class bHLH protein PTF1A; exocrine pancreas-specific transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; pancreas transcr |
| Accession | Q7RTS3 |
Fig.1. Human Pancreas transcription factor 1 subunit alpha (PTF1A) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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