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Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit

Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit

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Specification

Product name Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH1R in samples. An antibody specific for PTH1R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH1R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH1R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH1R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTH1R; MGC138426; MGC138452; PFE; PTHR; PTHR1; OTTHUMP00000209970; OTTHUMP00000209971; PTH/PTHr receptor; PTH/PTHrP type I receptor; parathyroid hormone receptor 1; parathyroid hormone/parathyroid hormone-relat

Product Properties

Kit components • Human Parathyroid Pormone 1 Receptor microplate
• Human Parathyroid Pormone 1 Receptor standard
• Human Parathyroid Pormone 1 Receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Parathyroid Pormone 1 Receptor (PTH1R) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTH1R. No significant cross-reactivity or interference between Human PTH1R and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Parathyroid hormone receptor 1 functions as a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH).This "classical" PTH receptor is expressed in high levels in bone and kidney and regulates calcium ion homeostasis through activation of adenylate cyclase and phospholipase C. In bone, it is expressed on the surface of osteoblasts. When the receptor is activated, these cells in turn stimulate osteoclasts to ultimately increase the resorption rate.It is a member of the secretin family of G protein-coupled receptors. The activity of this receptor is mediated by Gs G proteins which activate adenylyl cyclase. Besides, they also activate phosphatidylinositol-calcium second messenger system.Expressed in most tissues. Most abundant in kidney, bone and liver.
Gene ID 5745
Alternative PTH1R; MGC138426; MGC138452; PFE; PTHR; PTHR1; OTTHUMP00000209970; OTTHUMP00000209971; PTH/PTHr receptor; PTH/PTHrP type I receptor; parathyroid hormone receptor 1; parathyroid hormone/parathyroid hormone-relat
Accession Q03431

Image & description

Fig.1. Human Parathyroid Pormone 1 Receptor (PTH1R) Standard Curve.

Fig.1. Human Parathyroid Pormone 1 Receptor (PTH1R) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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