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Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit

Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit

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Specification

Product name Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit employs a two-site sandwich ELISA to quantitate PTH2R in samples. An antibody specific for PTH2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTH2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTH2R is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTH2R bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTH2R; PTHR2; PTH2 receptor; parathyroid hormone receptor 2

Product Properties

Kit components • Human Parathyroid hormone 2 receptor microplate
• Human Parathyroid hormone 2 receptor standard
• Human Parathyroid hormone 2 receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Parathyroid hormone 2 receptor (PTH2R) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTH2R. No significant cross-reactivity or interference between Human PTH2R and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Parathyroid hormone receptor 2 is a protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH). This receptor is more selective in ligand recognition and has a more specific tissue distribution compared to parathyroid hormone receptor 1 (PTHR1). There is 30 to 70% amino acid sequence identity between receptors within the secretin receptor family, but these receptors have essentially no sequence identity with most known G protein-coupled receptors of the rhodopsin family or with the metabotropic glutamate receptor family. PTHR2 is most similar (51% overall amino acid identity) to the PTH/PTHRP receptor. The PTHR2 gene was most abundantly expressed in brain, pancreas, testis, and placenta.
Gene ID 5746
Alternative PTH2R; PTHR2; PTH2 receptor; parathyroid hormone receptor 2
Accession P49190

Image & description

Fig.1. Human Parathyroid hormone 2 receptor (PTH2R) Standard Curve.

Fig.1. Human Parathyroid hormone 2 receptor (PTH2R) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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