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Human P2Y purinoceptor 12 (P2RY12) ELISA Kit

Human P2Y purinoceptor 12 (P2RY12) ELISA Kit

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Specification

Product name Human P2Y purinoceptor 12 (P2RY12) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human P2Y purinoceptor 12 (P2RY12) ELISA Kit employs a two-site sandwich ELISA to quantitate P2RY12 in samples. An antibody specific for P2RY12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP2RY12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P2RY12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P2RY12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative P2RY12; ADPG-R; HORK3; P2T(AC); P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999; ADP-glucose receptor; G-protein coupled receptor SP1999; Gi-coupled ADP receptor HORK3; platelet ADP receptor; purinergic receptor P2R

Product Properties

Kit components • Human P2Y purinoceptor 12 microplate
• Human P2Y purinoceptor 12 standard
• Human P2Y purinoceptor 12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human P2Y purinoceptor 12 (P2RY12) ELISA Kit has high sensitivity and excellent specificity for detection of Human P2RY12. No significant cross-reactivity or interference between Human P2RY12 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background In the field of molecular biology, the P2Y12 protein is found on the surface of blood platelet cells and is an important regulator in blood clotting. P2RY12belongs to a group of G protein-coupled purinergic receptors and is a chemoreceptor for adenosine diphosphate (ADP). The P2Y family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene.
Gene ID 64805
Alternative P2RY12; ADPG-R; HORK3; P2T(AC); P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999; ADP-glucose receptor; G-protein coupled receptor SP1999; Gi-coupled ADP receptor HORK3; platelet ADP receptor; purinergic receptor P2R
Accession Q9H244

Image & description

Fig.1. Human P2Y purinoceptor 12 (P2RY12) Standard Curve.

Fig.1. Human P2Y purinoceptor 12 (P2RY12) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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