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Human P2Y purinoceptor 1 (P2RY1) ELISA Kit

Human P2Y purinoceptor 1 (P2RY1) ELISA Kit

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Specification

Product name Human P2Y purinoceptor 1 (P2RY1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human P2Y purinoceptor 1 (P2RY1) ELISA Kit employs a two-site sandwich ELISA to quantitate P2RY1 in samples. An antibody specific for P2RY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyP2RY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for P2RY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of P2RY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative P2RY1; P2Y1; ATP receptor; P2 purinoceptor subtype Y1; P2Y purinoceptor 1; platelet ADP receptor; purinergic receptor P2Y1

Product Properties

Kit components • Human P2Y purinoceptor 1 microplate
• Human P2Y purinoceptor 1 standard
• Human P2Y purinoceptor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human P2Y purinoceptor 1 (P2RY1) ELISA Kit has high sensitivity and excellent specificity for detection of Human P2RY1. No significant cross-reactivity or interference between Human P2RY1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background P2RY1 belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation. The pharmacologic properties of the P2Y1 receptor are similar to those of the P2T ADP receptor that is responsible for platelet aggregation. They showed that the P2Y1 receptor is expressed by human platelets and megakaryoblasts. The authors suggested that the P2Y1 receptor may be the P2T receptor.
Gene ID 5028
Alternative P2RY1; P2Y1; ATP receptor; P2 purinoceptor subtype Y1; P2Y purinoceptor 1; platelet ADP receptor; purinergic receptor P2Y1
Accession P47900

Image & description

Fig.1. Human P2Y purinoceptor 1 (P2RY1) Standard Curve.

Fig.1. Human P2Y purinoceptor 1 (P2RY1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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