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Human P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit

Human P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit

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Specification

Product name Human P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit employs a two-site sandwich ELISA to quantitate SELPLG in samples. An antibody specific for SELPLG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySELPLG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SELPLG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SELPLG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SELPLG; CD162; CLA; PSGL-1; PSGL1; cutaneous lymphocyte-associated associated antigen

Product Properties

Kit components • Human P-selectin glycoprotein ligand 1 microplate
• Human P-selectin glycoprotein ligand 1 standard
• Human P-selectin glycoprotein ligand 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human P-selectin glycoprotein ligand 1 (SELPLG) ELISA Kit has high sensitivity and excellent specificity for detection of Human SELPLG. No significant cross-reactivity or interference between Human SELPLG and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters. P-selectin glycoprotein ligand-1 is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet). It is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte). PSGL-1 can bind to all three members of the family but binds best (with the highest affinity) to P-selectin.
Gene ID 6404
Alternative SELPLG; CD162; CLA; PSGL-1; PSGL1; cutaneous lymphocyte-associated associated antigen
Accession Q14242

Image & description

Fig.1. Human P-selectin glycoprotein ligand 1 (SELPLG) Standard Curve.

Fig.1. Human P-selectin glycoprotein ligand 1 (SELPLG) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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