|Product name||Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit|
|Applications notes||This Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit employs a two-site sandwich ELISA to quantitate TNK1 in samples. An antibody specific for TNK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Non-receptor tyrosine-protein kinase TNK1 microplate
• Human Non-receptor tyrosine-protein kinase TNK1 standard
• Human Non-receptor tyrosine-protein kinase TNK1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNK1. No significant cross-reactivity or interference between Human TNK1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||TNK1 is a nonreceptor tyrosine kinase. These kinases, like members of the SRC and JAK families, mediate intracellular signaling downstream of receptor activation. TNK1 contains a putative N-terminal phosphotyrosine-binding domain, followed by the tyrosine kinase domain, a putative SH3 domain, and a proline-rich C terminus that contains SH3-binding motifs. The kinase domain has all 12 structural features conserved in tyrosine kinases, including an ATP-binding site. TNK1 shares highest homology with ACK, including 45% amino acid identity over a 413-amino acid span that includes the kinase domain and putative SH3 domain. specific expression of 4 transcripts between 1.0 and 9.5 kb in fetal brain, lung, liver, and kidney.|
Fig.1. Human Non-receptor tyrosine-protein kinase TNK1 (TNK1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.