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Human Neuron navigator 1 (NAV1) ELISA Kit

Human Neuron navigator 1 (NAV1) ELISA Kit

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Specification

Product name Human Neuron navigator 1 (NAV1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Neuron navigator 1 (NAV1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAV1 in samples. An antibody specific for NAV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NAV1; DKFZp781D0314; FLJ12560; FLJ14203; KIAA1151; MGC14961; POMFIL3; STEERIN1; UNC53H1; neuron navigator-1; pore membrane and/or filament interacting like protein 3; unc-53 homolog 1

Product Properties

Kit components • Human Neuron navigator 1 microplate
• Human Neuron navigator 1 standard
• Human Neuron navigator 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Neuron navigator 1 (NAV1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAV1. No significant cross-reactivity or interference between Human NAV1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NAV1 belongs to the neuron navigator family and is expressed predominantly in the nervous system. Neuron navigator 1 contains coiled-coil domains and a conserved AAA domain characteristic for ATPases associated with a variety of cellular activities. NAV1 is similar to unc-53, a Caenorhabditis elegans gene involved in axon guidance. The exact function of NAV1 is not known, but it is thought to play a role in in neuronal development and regeneration. Alternative splicing results in multiple transcript variants.
Gene ID 89796
Alternative NAV1; DKFZp781D0314; FLJ12560; FLJ14203; KIAA1151; MGC14961; POMFIL3; STEERIN1; UNC53H1; neuron navigator-1; pore membrane and/or filament interacting like protein 3; unc-53 homolog 1
Accession Q8NEY1

Image & description

Fig.1. Human Neuron navigator 1 (NAV1) Standard Curve.

Fig.1. Human Neuron navigator 1 (NAV1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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