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Human Nanos homolog 1 (NANOS1) ELISA Kit

Human Nanos homolog 1 (NANOS1) ELISA Kit

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Specification

Product name Human Nanos homolog 1 (NANOS1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Nanos homolog 1 (NANOS1) ELISA Kit employs a two-site sandwich ELISA to quantitate NANOS1 in samples. An antibody specific for NANOS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNANOS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NANOS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NANOS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative NANOS1; NOS1; nanos homolog 1

Product Properties

Kit components • Human Nanos homolog 1 microplate
• Human Nanos homolog 1 standard
• Human Nanos homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Nanos homolog 1 (NANOS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NANOS1. No significant cross-reactivity or interference between Human NANOS1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background NANOS1 encodes a CCHC-type zinc finger protein that is a member of the nanos family. Nanos homolog 1 co-localizes with the RNA-binding protein pumilio RNA-binding family member 2 and may be involved in regulating translation as a post-transcriptional repressor. Mutations in NANOS1 are associated with spermatogenic impairment.
Gene ID 340719
Alternative NANOS1; NOS1; nanos homolog 1
Accession Q8WY41

Image & description

Fig.1. Human Nanos homolog 1 (NANOS1) Standard Curve.

Fig.1. Human Nanos homolog 1 (NANOS1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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