| Product name | Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) ELISA Kit employs a two-site sandwich ELISA to quantitate SIRT5 in samples. An antibody specific for SIRT5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT5 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SIRT5; FLJ36950; SIR2L5; silent mating type information regulation 2; S.cerevisiae; homolog 5; sir2-like 5; sirtuin 5; sirtuin type 5 |
| Kit components | • Human NAD-dependent deacetylase sirtuin-5 microplate • Human NAD-dependent deacetylase sirtuin-5 standard • Human NAD-dependent deacetylase sirtuin-5 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIRT5. No significant cross-reactivity or interference between Human SIRT5 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | SIRT5 encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in two transcript variants. |
| Gene ID | 23408 |
| Alternative | SIRT5; FLJ36950; SIR2L5; silent mating type information regulation 2; S.cerevisiae; homolog 5; sir2-like 5; sirtuin 5; sirtuin type 5 |
| Accession | Q9NXA8 |
Fig.1. Human NAD-dependent deacetylase sirtuin-5 (SIRT5/SIR2L5) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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