Login Register
English
0

Cart

$ 0

Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit

Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit

Views(68) Publications(0) Catalog no(KTE61444)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12B in samples. An antibody specific for MYL12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYL12B; MLC-B; MRLC2; OTTHUMP00000165808; myosin regulatory light chain 2; myosin regulatory light chain MRCL2; myosin regulatory light chain MRLC2

Product Properties

Kit components • Human Myosin regulatory light chain 12B microplate
• Human Myosin regulatory light chain 12B standard
• Human Myosin regulatory light chain 12B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYL12B. No significant cross-reactivity or interference between Human MYL12B and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MYL12B (Myosin Light Chain 12B) is a Protein Coding gene. Among its related pathways are Semaphorin interactions and Tight junction. GO annotations related to this gene include calcium ion binding. An important paralog of this gene is MYL12A.
Gene ID 614055
Alternative MYL12B; MLC-B; MRLC2; OTTHUMP00000165808; myosin regulatory light chain 2; myosin regulatory light chain MRCL2; myosin regulatory light chain MRLC2
Accession A4IF97

Image & description

Fig.1. Human Myosin regulatory light chain 12B (MYL12B) Standard Curve.

Fig.1. Human Myosin regulatory light chain 12B (MYL12B) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Myosin regulatory light chain 12B (MYL12B) ELISA Kit”