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Human Myosin-IIIa (MYO3A) ELISA Kit

Human Myosin-IIIa (MYO3A) ELISA Kit

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Product name Human Myosin-IIIa (MYO3A) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Myosin-IIIa (MYO3A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO3A in samples. An antibody specific for MYO3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO3A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYO3A; DFNB30;

Product Properties

Kit components • Human Myosin-IIIa microplate
• Human Myosin-IIIa standard
• Human Myosin-IIIa detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Myosin-IIIa (MYO3A) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO3A. No significant cross-reactivity or interference between Human MYO3A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Myosin-IIIa encoded by MYO3A belongs to the myosin superfamily. Myosins are actin-dependent motor proteins and are categorized into conventional myosins (class II) and unconventional myosins (classes I and III through XV) based on their variable C-terminal cargo-binding domains. Class III myosins, such as this one, have a kinase domain N-terminal to the conserved N-terminal motor domains and are expressed in photoreceptors. Myosin-IIIa encoded by MYO3A plays an important role in hearing in humans. Three different recessive, loss of function mutations in the encoded protein have been shown to cause nonsyndromic progressive hearing loss. Expression of MYO3A is highly restricted, with the strongest expression in retina and cochlea.
Gene ID 53904
Alternative MYO3A; DFNB30;
Accession Q8NEV4

Image & description

Fig.1. Human Myosin-IIIa (MYO3A) Standard Curve.

Fig.1. Human Myosin-IIIa (MYO3A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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