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Human Myosin-Id (MYO1D) ELISA Kit

Human Myosin-Id (MYO1D) ELISA Kit

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Product name Human Myosin-Id (MYO1D) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Myosin-Id (MYO1D) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO1D in samples. An antibody specific for MYO1D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO1D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO1D is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO1D bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MYO1D; KIAA0727; myr4; myosin-I gamma

Product Properties

Kit components • Human Myosin-Id microplate
• Human Myosin-Id standard
• Human Myosin-Id detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Myosin-Id (MYO1D) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO1D. No significant cross-reactivity or interference between Human MYO1D and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MYO1D (Myosin ID) is a Protein Coding gene. Diseases associated with MYO1D include Visceral Heterotaxy. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Delta508-CFTR traffic / ER-to-Golgi in CF. GO annotations related to this gene include actin binding and calmodulin binding. An important paralog of this gene is MYO1G. Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity).
Gene ID 4642
Alternative MYO1D; KIAA0727; myr4; myosin-I gamma
Accession O94832

Image & description

Fig.1. Human Myosin-Id (MYO1D) Standard Curve.

Fig.1. Human Myosin-Id (MYO1D) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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