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Human Mucin 2 (MUC2) ELISA Kit

Human Mucin 2 (MUC2) ELISA Kit

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Specification

Product name Human Mucin 2 (MUC2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mucin 2 (MUC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC2 in samples. An antibody specific for MUC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MUC2; MLP; SMUC; mucin 2; mucin 2; intestinal/tracheal; mucin-like protein

Product Properties

Kit components • Human Mucin 2 microplate
• Human Mucin 2 standard
• Human Mucin 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mucin 2 (MUC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MUC2. No significant cross-reactivity or interference between Human MUC2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MUC2 encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by MUC2 is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Downregulation of MUC2 has been observed in patients with Crohn disease and ulcerative colitis.
Alternative MUC2; MLP; SMUC; mucin 2; mucin 2; intestinal/tracheal; mucin-like protein
Accession Q02817

Image & description

Fig.1. Human Mucin 2 (MUC2) Standard Curve.

Fig.1. Human Mucin 2 (MUC2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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