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Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit

Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit

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Specification

Product name Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit employs a two-site sandwich ELISA to quantitate SMAD4 in samples. An antibody specific for SMAD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SMAD4; DPC4; JIP; MADH4; MAD; mothers against decapentaplegic homolog 4; OTTHUMP00000163548; SMAD; mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; mothers against decapentaplegic homolo

Product Properties

Kit components • Human Mothers against decapentaplegic homolog 4 microplate
• Human Mothers against decapentaplegic homolog 4 standard
• Human Mothers against decapentaplegic homolog 4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mothers against decapentaplegic homolog 4 (SMAD4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SMAD4. No significant cross-reactivity or interference between Human SMAD4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SMAD4 is a 552 amino acid protein involved in cell signaling. It belongs to the Darfwin family of proteins which modulate members of the TGF beta protein superfamily. It binds receptor regulated SMADs such as SMAD1 or SMAD2 and forms a complex that binds to DNA and serves as a transcription factor. It is the only known mammalian coSMAD. It is a homolog of the Drosophila protein: "Mothers against decapentaplegic". The SMAD proteins are homologs of both the drosophila protein, mothers against decapentaplegic (MAD) and the C. elegans protein SMA. The name is a combination of the two. During Drosophila research, it was found that a mutation in the gene, MAD, in the mother, repressed the gene, decapentaplegic, in the embryo.
Gene ID 4089
Alternative SMAD4; DPC4; JIP; MADH4; MAD; mothers against decapentaplegic homolog 4; OTTHUMP00000163548; SMAD; mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; mothers against decapentaplegic homolo
Accession Q13485

Image & description

Fig.1. Human Mothers against decapentaplegic homolog 4 (SMAD4) Standard Curve.

Fig.1. Human Mothers against decapentaplegic homolog 4 (SMAD4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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