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Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit

Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit

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Specification

Product name Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM40 in samples. An antibody specific for TOMM40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM40 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TOMM40; C19orf1; D19S1177E; PER-EC1; PEREC1; TOM40; mitochondrial outer membrane protein; translocase of outer mitochondrial membrane 40

Product Properties

Kit components • Human Mitochondrial import receptor subunit TOM40 homolog microplate
• Human Mitochondrial import receptor subunit TOM40 homolog standard
• Human Mitochondrial import receptor subunit TOM40 homolog detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOMM40. No significant cross-reactivity or interference between Human TOMM40 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TOMM40 is the channel-forming subunit of the translocase of the mitochondrial outer membrane (TOM) complex that is essential for protein import into mitochondria.The deduced protein contains 361 amino acids. Fractionation of rat liver detected Tomm40 within the mitochondrial fraction. Extraction and antibody-accessibility studies suggested that Tomm40 is embedded within the mitochondrial membrane.Tomm40 was associated with a 400-kD complex containing the mitochondrial import receptors Tom20 and Tom22 and several smaller components. Mitochondria-targeted preproteins interacted with the Tomm40-containing complex during their transit to the mitochondrial matrix, and immunodepletion of Tomm40 caused the loss of preprotein import activity.
Gene ID 10452
Alternative TOMM40; C19orf1; D19S1177E; PER-EC1; PEREC1; TOM40; mitochondrial outer membrane protein; translocase of outer mitochondrial membrane 40
Accession O96008

Image & description

Fig.1. Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) Standard Curve.

Fig.1. Human Mitochondrial import receptor subunit TOM40 homolog (TOMM40) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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