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Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit

Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit

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Specification

Product name Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit employs a two-site sandwich ELISA to quantitate MAST3 in samples. An antibody specific for MAST3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAST3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAST3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAST3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MAST3; KIAA0561;

Product Properties

Kit components • Human Microtubule-associated serine/threonine-protein kinase 3 microplate
• Human Microtubule-associated serine/threonine-protein kinase 3 standard
• Human Microtubule-associated serine/threonine-protein kinase 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAST3. No significant cross-reactivity or interference between Human MAST3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background By sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1998) cloned MAST3, which they designated KIAA0561. The transcript contains repetitive elements in its 3-prime UTR, and the deduced 1,308-amino acid protein shares significant similarity with mouse Mast205 (MAST2) and human MAST4. RT-PCR detected variable MAST3 expression in all tissues examined, with highest levels in kidney, brain, and small intestine.Valiente et al. (2005) showed that the C-terminal tail of human PTEN bound to the PDZ domains of rat Magi2, Magi3, and Dlg (DLG1), mouse Sast (MAST1) and Mast205, and human MAST3. Interaction of PTEN with Magi2 increased PTEN protein stability, and interaction of PTEN with the MAST kinases facilitated phosphorylation of PTEN by these kinases.
Gene ID 23031
Alternative MAST3; KIAA0561;
Accession O60307

Image & description

Fig.1. Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) Standard Curve.

Fig.1. Human Microtubule-associated serine/threonine-protein kinase 3 (MAST3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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