| Product name | Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MCCC1 in samples. An antibody specific for MCCC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCCC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCCC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCCC1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MCCC1; DKFZp686B20267; FLJ25545; MCC-B; MCCA; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha |
| Kit components | • Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial microplate • Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial standard • Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MCCC1. No significant cross-reactivity or interference between Human MCCC1 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The MCCD1 gene encodes a deduced 119-amino acid protein containing a putative coiled-coil domain at its C terminus and a predicted mitochondrial localization signal at its N terminus. RT-PCR analysis detected expression of MCCD1 in all human tissues tested, at highest levels in adult and fetal kidney, liver and lung, and fetal brain, and at lower levels in fetal spleen. The human and pig proteins share 65.9% overall sequence identity but their C-terminal domains are highly conserved, showing 92% identity over 53 residues. No orthologs were identified in any other species, suggesting that the gene arose relatively recently in evolution. Transient expression in mammalian cells of MCCD1 fused at its C terminus to either EGFP or the T7-epitope tag showed that the protein is localized to mitochondria. |
| Gene ID | 56922 |
| Alternative | MCCC1; DKFZp686B20267; FLJ25545; MCC-B; MCCA; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha |
| Accession | Q96RQ3 |
Fig.1. Human Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial (MCCC1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
You must be logged in to post a review.
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
Welcome any form of communications, and better service will be provided here.
Tell： +1-404-854-0155
Email： service@abbkine.com
Support Email： support@abbkine.com
Address： 3052 Stroop Hill Road, Apt 203, Atlanta 30303, Georgia, United States of America
Reviews
There are no reviews yet.