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Human Metallothionein-3 (MT3) ELISA Kit

Human Metallothionein-3 (MT3) ELISA Kit

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Product name Human Metallothionein-3 (MT3) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Metallothionein-3 (MT3) ELISA Kit employs a two-site sandwich ELISA to quantitate MT3 in samples. An antibody specific for MT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MT3; GIF; GIFB; GRIF; metallothionein 3 (growth inhibitory factor (neurotrophic))

Product Properties

Kit components • Human Metallothionein-3 microplate
• Human Metallothionein-3 standard
• Human Metallothionein-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Metallothionein-3 (MT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human MT3. No significant cross-reactivity or interference between Human MT3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MT3 (Metallothionein 3) is a Protein Coding gene, and is affiliated with the lncRNA class. Diseases associated with MT3 include Alzheimer Disease and Amyotrophic Lateral Sclerosis 1. Among its related pathways are Metabolism and Response to metal ions. GO annotations related to this gene include drug binding and antioxidant activity. Binds heavy metals. Contains three zinc and three copper atoms per polypeptide chain and only a negligible amount of cadmium. Inhibits survival and neurite formation of cortical neurons in vitro.
Gene ID 4504
Alternative MT3; GIF; GIFB; GRIF; metallothionein 3 (growth inhibitory factor (neurotrophic))
Accession P25713

Image & description

Fig.1. Human Metallothionein-3 (MT3) Standard Curve.

Fig.1. Human Metallothionein-3 (MT3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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