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Human Mesoderm posterior protein 2 (MESP2) ELISA Kit

Human Mesoderm posterior protein 2 (MESP2) ELISA Kit

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Specification

Product name Human Mesoderm posterior protein 2 (MESP2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Mesoderm posterior protein 2 (MESP2) ELISA Kit employs a two-site sandwich ELISA to quantitate MESP2 in samples. An antibody specific for MESP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMESP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MESP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MESP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MESP2; SCDO2; bHLHc6; mesoderm posterior 2 homolog

Product Properties

Kit components • Human Mesoderm posterior protein 2 microplate
• Human Mesoderm posterior protein 2 standard
• Human Mesoderm posterior protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Mesoderm posterior protein 2 (MESP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MESP2. No significant cross-reactivity or interference between Human MESP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MESP2 encodes a member of the bHLH family of transcription factors and plays a key role in defining the rostrocaudal patterning of somites via interactions with multiple Notch signaling pathways. MESP2 is expressed in the anterior presomitic mesoderm and is downregulated immediately after the formation of segmented somites. MESP2 also plays a role in the formation of epithelial somitic mesoderm and cardiac mesoderm. Mutations in the MESP2 gene cause autosomal recessive spondylocostal dystosis 2 (SCD02).
Gene ID 145873
Alternative MESP2; SCDO2; bHLHc6; mesoderm posterior 2 homolog
Accession Q0VG99

Image & description

Fig.1. Human Mesoderm posterior protein 2 (MESP2) Standard Curve.

Fig.1. Human Mesoderm posterior protein 2 (MESP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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