| Product name | Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit employs a two-site sandwich ELISA to quantitate MED16 in samples. An antibody specific for MED16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED16 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | MED16; DRIP92; THRAP5; TRAP95; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein; 95-kD subunit |
| Kit components | • Human Mediator of RNA polymerase II transcription subunit 16 microplate • Human Mediator of RNA polymerase II transcription subunit 16 standard • Human Mediator of RNA polymerase II transcription subunit 16 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Mediator of RNA polymerase II transcription subunit 16 (MED16) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED16. No significant cross-reactivity or interference between Human MED16 and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MED16 (Mediator Complex Subunit 16) is a Protein Coding gene. Among its related pathways are Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha) and Metabolism. GO annotations related to this gene include receptor activity and transcription cofactor activity. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Gene ID | 10025 |
| Alternative | MED16; DRIP92; THRAP5; TRAP95; thyroid hormone receptor-associated protein 5; thyroid hormone receptor-associated protein; 95-kD subunit |
| Accession | Q9Y2X0 |
Fig.1. Human Mediator of RNA polymerase II transcription subunit 16 (MED16) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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