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Human Max-binding protein MNT (MNT) ELISA Kit

Human Max-binding protein MNT (MNT) ELISA Kit

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Specification

Product name Human Max-binding protein MNT (MNT) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Max-binding protein MNT (MNT) ELISA Kit employs a two-site sandwich ELISA to quantitate MNT in samples. An antibody specific for MNT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMNT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MNT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MNT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MNT; MAD6; MXD6; ROX; bHLHd3; Max-interacting protein; myc antagonist

Product Properties

Kit components • Human Max-binding protein MNT microplate
• Human Max-binding protein MNT standard
• Human Max-binding protein MNT detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Max-binding protein MNT (MNT) ELISA Kit has high sensitivity and excellent specificity for detection of Human MNT. No significant cross-reactivity or interference between Human MNT and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. MNT encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain.
Gene ID 4335
Alternative MNT; MAD6; MXD6; ROX; bHLHd3; Max-interacting protein; myc antagonist
Accession Q99583

Image & description

Fig.1. Human Max-binding protein MNT (MNT) Standard Curve.

Fig.1. Human Max-binding protein MNT (MNT) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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