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Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit

Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit

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Specification

Product name Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit employs a two-site sandwich ELISA to quantitate MPHOSPH6 in samples. An antibody specific for MPHOSPH6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPHOSPH6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPHOSPH6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPHOSPH6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MPHOSPH6; MPP; MPP-6; MPP6;

Product Properties

Kit components • Human M-phase phosphoprotein 6 microplate
• Human M-phase phosphoprotein 6 standard
• Human M-phase phosphoprotein 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human M-phase phosphoprotein 6 (MPHOSPH6) ELISA Kit has high sensitivity and excellent specificity for detection of Human MPHOSPH6. No significant cross-reactivity or interference between Human MPHOSPH6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MPHOSPH6 (M-Phase Phosphoprotein 6) is a Protein Coding gene. Among its related pathways are rRNA processing in the nucleus and cytosol and Deadenylation-dependent mRNA decay. GO annotations related to this gene include RNA binding. RNA-binding protein that associates with the RNA exosome complex. Involved in the 3-processing of the 7S pre-RNA to the mature 5.8S rRNA and may play a role in recruiting the RNA exosome complex to pre-rRNA; this function may include C1D.
Gene ID 10200
Alternative MPHOSPH6; MPP; MPP-6; MPP6;
Accession Q99547

Image & description

Fig.1. Human M-phase phosphoprotein 6 (MPHOSPH6) Standard Curve.

Fig.1. Human M-phase phosphoprotein 6 (MPHOSPH6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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