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Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) ELISA Kit

Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) ELISA Kit

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Specification

Product name Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) ELISA Kit employs a two-site sandwich ELISA to quantitate LPCAT4 in samples. An antibody specific for LPCAT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLPCAT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPCAT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPCAT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative LPCAT4; AGPAT7; AYTL3; FLJ10257; LPAAT-eta; LPEAT2; 1-acylglycerol-3-phosphate O-acyltransferase 7 (lysophosphatidic acid acyltransferase; eta); PLSC domain containing protein; acyl-CoA:lysophosphatidylethano

Product Properties

Kit components • Human Lysophospholipid acyltransferase LPCAT4 microplate
• Human Lysophospholipid acyltransferase LPCAT4 standard
• Human Lysophospholipid acyltransferase LPCAT4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) ELISA Kit has high sensitivity and excellent specificity for detection of Human LPCAT4. No significant cross-reactivity or interference between Human LPCAT4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Members of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family, such as AGPAT7, catalyze the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction.The deduced 524-amino acid protein has a calculated molecular mass of 57.2 kD. It has a phosphate acyltransferase domain that shares 15 to 29% amino acid identity with the acyltransferase domains of other AGPATS and contains all the residues essential for acyltransferase activity. AGPAT7 expression in uterus, thymus, pancreas, skeletal muscle, bladder, stomach, lung, and testis. Fluorescence-tagged AGPAT7 showed a perinuclear and punctate distribution in transfected HeLa cells and colocalized with an endoplasmic reticulum marker.
Gene ID 254531
Alternative LPCAT4; AGPAT7; AYTL3; FLJ10257; LPAAT-eta; LPEAT2; 1-acylglycerol-3-phosphate O-acyltransferase 7 (lysophosphatidic acid acyltransferase; eta); PLSC domain containing protein; acyl-CoA:lysophosphatidylethano
Accession Q643R3

Image & description

Fig.1. Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) Standard Curve.

Fig.1. Human Lysophospholipid acyltransferase LPCAT4 (LPCAT4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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