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Human Leukosialin (SPN) ELISA Kit

Human Leukosialin (SPN) ELISA Kit

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Product name Human Leukosialin (SPN) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Leukosialin (SPN) ELISA Kit employs a two-site sandwich ELISA to quantitate SPN in samples. An antibody specific for SPN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPN; CD43; GPL115; LSN; leukosialin; sialophorin (gpL115; leukosialin; CD43); sialophorin (leukosialin; CD43)

Product Properties

Kit components • Human Leukosialin microplate
• Human Leukosialin standard
• Human Leukosialin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Leukosialin (SPN) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPN. No significant cross-reactivity or interference between Human SPN and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.The nucleotide sequence of the 1.7-kb clone showed that the sialophorin transcript has a 3-prime-noncoding region of 587 nucleotides, is polyadenylated, and has the AATAAA polyadenylation signal. The derived sequence of 341 amino acids showed that the sialophorin protein has 3 domains: an extracellular domain of 195 amino acids, a hydrophobic transmembrane domain of 23 amino acids, and a C-terminal intracellular domain of 123 amino acids which contains a number of sites that might be phosphorylated by protein kinase C.
Gene ID 6693
Alternative SPN; CD43; GPL115; LSN; leukosialin; sialophorin (gpL115; leukosialin; CD43); sialophorin (leukosialin; CD43)
Accession P16150

Image & description

Fig.1. Human Leukosialin (SPN) Standard Curve.

Fig.1. Human Leukosialin (SPN) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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