| Product name | Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | This Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit employs a two-site sandwich ELISA to quantitate LRRC4B in samples. An antibody specific for LRRC4B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLRRC4B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRC4B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRC4B bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | LRRC4B; DKFZp761A179; HSM; LRIG4; leucine-rich repeats and immunoglobulin-like domains 4 |
| Kit components | • Human Leucine-rich repeat-containing protein 4B microplate • Human Leucine-rich repeat-containing protein 4B standard • Human Leucine-rich repeat-containing protein 4B detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Human Leucine-rich repeat-containing protein 4B (LRRC4B) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRRC4B. No significant cross-reactivity or interference between Human LRRC4B and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | LRRC4B is a synaptic adhesion protein. It regulates the formation of excitatory synapses. The trans-synaptic adhesion between LRRC4B and LAR regulates excitatory synapse formation in a bidirectional manner. LRRC4 are post-synaptic adhesion molecules of the LRR protein family that induce excitatory synapse formation. Mature LRRC4 family members are type I transmembrane proteins with an extracellular region that contains nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. LRRC4 family members share ~55% aa identity, but each recognizes different ligands. LRRC4 and LRRC4C recognize Netrin-G2 and Netrin-G3, respectively, while LRRC4B recognizes the receptor tyrosine phosphatases LAR/PTPRF, PTPR delta and PTPR sigma. |
| Gene ID | 94030 |
| Alternative | LRRC4B; DKFZp761A179; HSM; LRIG4; leucine-rich repeats and immunoglobulin-like domains 4 |
| Accession | Q9NT99 |
Fig.1. Human Leucine-rich repeat-containing protein 4B (LRRC4B) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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