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Human Inactive serine protease 35 (PRSS35) ELISA Kit

Human Inactive serine protease 35 (PRSS35) ELISA Kit

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Specification

Product name Human Inactive serine protease 35 (PRSS35) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Inactive serine protease 35 (PRSS35) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS35 in samples. An antibody specific for PRSS35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS35 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS35 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS35 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRSS35; C6orf158; MGC46520; dJ223E3.1; inactive serine protease 35

Product Properties

Kit components • Human Inactive serine protease 35 microplate
• Human Inactive serine protease 35 standard
• Human Inactive serine protease 35 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Inactive serine protease 35 (PRSS35) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS35. No significant cross-reactivity or interference between Human PRSS35 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Proteolytic degradation of extracellular matrix components has been suggested to play an essential role for the occurrence of ovulation. The plasminogen activator and matrix metalloproteinase systems, which were previously believed to be crucial for ovulation, are not required in this process. PRSS35, which was upregulated by gonadotropins. PRSS23 was highly expressed in atretic follicles and it was expressed in the ovarian stroma and theca tissues just prior to ovulation. PRSS35 was expressed in the theca layers of developing follicles. It was also highly induced in granulosa cells of preovulatory follicles. PRSS35 was also expressed in the forming and regressing CL. PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia.
Gene ID 167681
Alternative PRSS35; C6orf158; MGC46520; dJ223E3.1; inactive serine protease 35
Accession Q8N3Z0

Image & description

Fig.1. Human Inactive serine protease 35 (PRSS35) Standard Curve.

Fig.1. Human Inactive serine protease 35 (PRSS35) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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