| Product name | Human IL-4 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IL-4 (His25-Ser153) (P05112) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL.The specific activity of recombinant human IL-4 is approximately >2.8 x 10⁷ IU/mg. |
| Protein length | The recombinant Human IL-4 Protein consists of 129 amino acids and predicts a molecular mass of 12.3 kDa. The protein migrates as 12 kDa under reducing condition (SDS-PAGE analysis). |
| Preparation method | E. coli |
| Purity | > 98% as determined by SDS-PAGE. |
| Alternative | BCGF, BCDF, B-cell Stimulating Factor (BSF-1) |
| Formulation | Lyophilized from sterile PBS, pH 8.0 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 15.9 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-4 (IL-4) is a multipotent cytokine involved in the regulation of immune response. IL-4 is secreted mainly by mast cells, T cells, eosinophils and basophils, and is the receptor of IL-4 RA. The effect of IL-4 depends on the type of shared receptor gamma chain (γc) that its receptor IL-4 RA recruits. IL-2Rγ is a common subunit of several interleukin receptors. IL-4 RArecruits IL-2Rγ and forms a Type I signaling complex with IL-4 expressed on hematopoietic cells, leading to a series of receptor-related kinase-mediated phosphorylation events. |
| Alternative | BCGF, BCDF, B-cell Stimulating Factor (BSF-1) |
| Accession | P05112 |
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