| Product name | Human IL-33 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IL-33 (Ser112-Thr270) (Q9HBE4) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measured by its ability to binding with recombinant ST2L (IL-33 receptor). The ED50 for this effect is <54 ng/mL. |
| Protein length | The recombinant Human IL-33 Protein consists of 159 amino acids and predicts a molecular mass of 18.9 kDa |
| Preparation method | E. coli |
| Purity | > 98% as determined by SDS-PAGE. |
| Alternative | IL-1 F11, NF-HEV, DVS27 |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 18.93 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-21 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin 33 (IL-33) is an 18 kDa cytokine with 169 amino acid residues. Interleukin-33 is a member of the IL-1 cytokine family and is primarily expressed in endothelial cells, epithelial cells, fibroblasts, and mesenchymal cells. When IL-33 binds to its receptor, ST2L, it activates mast cells and innate lymphoid cells and subsequently generates IL-5 and IL-13, which play essential roles in allergic inflammation. IL-33 also acts as an alarm by alerting the immune system of cellular damage and plays a critical role in type-2 innate immunity. |
| Alternative | IL-1 F11, NF-HEV, DVS27 |
| Accession | O95760 |
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