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Human IL-28A Protein, His tag (Animal-Free)

Human IL-28A Protein, His tag (Animal-Free)

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Specification

Product name Human IL-28A Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-28A (Pro27-Val200) (Q8IZJ0) was expressed with 6×His tag at the C-terminus.
Applications His tag
Applications notes Human
Activity Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <20 ng/mL.
Protein length The recombinant Human IL-28A consists of 174 amino acids and predicts a molecular mass of 20.6 kDa.
Preparation method E.coli
Purity > 98 % as determined by SDS-PAGE
Molecular Formula 20.60 kDa
Alternative interleukin 28A, IFN-λ2, IL28, IL28A

Product Properties

Formulation Lyophilized from sterile PBS,pH 8.0.
Features & Benefits <0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 20.60 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously.
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA,10% FBS or 0.1%BSA). Please prevent freeze-thaw cycles.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 28A (IL-28A) predicts a molecular mass of 22.3 kDa. IL-28 comes in two isoforms, IL-28A and IL-28B, and plays a role in immune defense against viruses, including the induction of an antiviral state by turning on Mx proteins, 2',5'-oligoadenylate synthetase as well as ISGF3G.
Gene ID 282616
Alternative interleukin 28A, IFN-λ2, IL28, IL28A
Accession Q8IZJ0

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