| Product name | Human IL-2 protein |
| Sequence | Amino acid sequence derived from human IL-2 isoform (P60568-1) (Ala21-Thr153) was expressed. |
| Activity | Measured by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is 2.269 ng/ml. |
| Protein length | The recombinant human IL-2 consists of 134 amino acids (N-Met) and has a predicted molecular mass of 15.5 KD. The apparent molecular mass of the human IL-2 is approximately 16 KD in SDS-PAGE under reducing conditions due to glycosylation. |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE |
| Alternative | Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2 |
| Formulation | Lyophilized from sterile 20 mM Tris, 50 mM NaCl, pH 5.0, 1 mM DTT, 1 mM EDTA. |
| Features & Benefits | Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method. |
| Molecular weight | 15.5 KD |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-2 protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage instructions | Lyophilized Human IL-2 proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.16% HSA or BSA). Please prevent freeze-thaw cycles. |
| Shipping | The product is shipped at ambient temperature. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin (IL-2) abbreviated as interleukin, refers to a lymphokine that interacts between leukocytes or immune cells, and it belongs to the same cytokine as blood cell growth factor. The two coordinate and interact with each other to complete hematopoietic and immune regulation functions together. Interleukins play an important role in transmitting information, activating and regulating immune cells, mediating the activation, proliferation and differentiation of T and B cells, and in inflammation. |
| Gene ID | 3558 |
| Alternative | Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2 |
| Accession | P60568 |
Fig. SDS-PAGE analysis of Human IL-2 protein.
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