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Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit

Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit

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Specification

Product name Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit employs a two-site sandwich ELISA to quantitate PTBP1 in samples. An antibody specific for PTBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTBP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTBP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTBP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTBP1; HNRNP-I; HNRNPI; HNRPI; MGC10830; MGC8461; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; pPTB; RNA-binding protein; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptid

Product Properties

Kit components • Human Heterogeneous nuclear ribonucleoprotein I microplate
• Human Heterogeneous nuclear ribonucleoprotein I standard
• Human Heterogeneous nuclear ribonucleoprotein I detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTBP1. No significant cross-reactivity or interference between Human PTBP1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background hnRNP I/PTB belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. Heterogeneous nuclear ribonucleoprotein I encoded by hnRNP I/PTB has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. Heterogeneous nuclear ribonucleoprotein I is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Gene ID 5725
Alternative PTBP1; HNRNP-I; HNRNPI; HNRPI; MGC10830; MGC8461; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; pPTB; RNA-binding protein; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptid
Accession P26599

Image & description

Fig.1. Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) Standard Curve.

Fig.1. Human Heterogeneous nuclear ribonucleoprotein I (hnRNP I/PTB) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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